reference strain造句
例句与造句
- The reference strain of bj were clustered into subgroup g independently
参比菌株bj独立聚为g亚群。 - Based on the result of numerical taxonomy , 16s rdna pcr - rflp were applied to 12 isolates and 9 reference strains
一些菌株如ccbau61116 、 ccbau41069 、 ccbau23168等具有专一的酶切图谱类型。 - And another nine strains and strains hclv , shimen of two major reference strains of china belonged to group i . in addition , strain p97 was the special individual
G皿z 、 gxnn 、与属1群的中国主要参考毒株hclv 、 shimen在遗传关系上较远，与italy株和paderborn株比较接近。 - Substitution and insertion in all strains si gene , the homogeneity of nucleotide and the deduced amino acids of s1 gene with 17 alien and domestic references strains were equally less than 80 %
S1基因除与qx的亲缘关系较近外，与其它参考株的同源率均低于80 ，可能为一株国内流行性的变异株。 - The cluster analysis on 126 phenotypic characteristics of 117strains isolated from fermented foods were proceeded , 110 strains are gram positive strains and 7 strains are reference strains as comparison
从新分离的和原来保藏的菌株中，选择了革兰氏阳性菌株110株和7株参比菌株，进行了菌株表型特性的测定。 - It's difficult to find reference strain in a sentence. 用reference strain造句挺难的
- Results of phenotype test shown that all peanut isolates and reference strains of b . japonicum and b . elkanii were clustered into a group and differed from the other genus of fast - growing rhizobia in low similarity
表型分析结果表明所有供试菌株与慢生参比菌株b . japonicum和b . elkanii聚为一群，而其它种属的参比菌株聚为另一群，表明花生根瘤菌在属的水平上应属于bradyrhizobium属。 - Relatively , the new wibdv strains gx8 / 99 had less homology to wibdv reference strain hk46 and other 3 field strains as 96 . 8 % - 97 . 2 % at dna or aa levels , than the homology among hk46 and 3 strains , strains gx8 / 99 more than 98 . 4 % - 98 . 6 % at dna or aa levels
为研究病毒的核酸分子结构与其致病性的关系，本研究选取了在致死率上不同的4个ibdv野毒株，比较了它们的vpz基因高变区共494个碱基序列。 - Were studied together with the reference strains of recognized rhizobium and bradyrhizobiwn species by performing polyphasic taxonomy , including numerical taxonomy , rep - pcr fingerprinting , 16s rdna pcr - rflp . the result show that : the growth rate of rhizobia isolated from the root nodules of pueraria spp . showed great diversity . ccbau41147 ccbau6110 k ccbau61096 and ccbau61095 were fast - growing strains , the single colony size was bigger than 1mm after 2 days incubated oq yma medium at 28 they can produce acid . the other strains were slow - growing strains , their single colony size was less than 1 mm after 7 days incubated on yma medium at 28 . they can produce alkali
本研究以从我国四川、河南、安徽和湖南等地分离的32株葛藤根瘤菌为研究对象，以20株已知种的根瘤菌为参比菌株，采用数值分类、 rep - pcr指纹分析、 16srdnapcr - rflp指纹分析等现代根瘤菌分类技术，初步研究了葛藤根瘤菌的生物多样性和分类地位，结果表明：葛藤根瘤菌在生长速率上表现出多样性，菌株ccbau41147 、 ccbau61096 、 ccbau61101和ccbau61095生长较快， yma培养基上28培养2 - 3天后，单个菌落直径大于1mm ，具有产酸能力，是快生型葛藤根瘤菌；其余待测葛藤根瘤菌生长较慢， yma培养基上28培养7天后，单个菌落直径小于1mm ，具有产碱能力，是慢生型葛藤根瘤菌。 - In this paper , first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv . the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector . after transforming e . coli dh5 a , ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr . presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc . comparing the aquired sequence of 3abc with that of reference strains , the homology is more than 99 percent . the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo . lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene , which happened to form a terminator codon behind 3ab gene , but it contained the complete open reading frame ( orf ) of 3ab gene . positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ) , bacteria were detected by sds - page and western blotting after properly treated . the results showed that the 3ab gene expressed successfully in e . coli and 33 . 5ku fusion protein can be recognized by the positive bovine serum of fmdv . the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
扩增产物连接到pgem - teasy载体中，转化大肠杆菌dh5菌株，筛选氨苄青霉素抗性菌落，提取质粒经酶切鉴定、 pcr分析以及确证性测序证明，所克隆的1500bp左右的片段含有完整的3abc基因，与国外参考序列相比，同源性在99以上。将重组质粒pgem - 3abc和表达载体ptriex - 4neo分别用sal和bgl与xho和bgl消化后，亚克隆3abc基因至原核表达载体ptriex - 4neo中，通过酶切鉴定、 pcr扩增以及序列分析，发现克隆到ptriex - 4neo载体上的片段于3abc基因708bp处出现了17bp的缺失，碰巧在3ab基因后形成一终止密码子，但3ab基因的阅读框架完整，选出含有3ab基因完整阅读框架的阳性克隆，用iptg诱导表达，收集菌液进行sds - page电泳、 westernblotting分析，结果表明， 3ab基因在大肠杆菌中成功表达，其表达产物为分子量33 . 5ku的融合蛋白，并能被口蹄疫病毒阳性血清识别。经薄层扫描分析，表达量占总蛋白量的26以上。 - The results indicate that the nucleotide sequences and deduced amino acid sequences of all the guangxi isolates in the signal peptides were highly homologous , but lowly homologous with other reference strains . the amino acid composes and arrangement of all guangxi isolates at the cleavage site has the typical pattern of ndv virulent strains , and is identical with the facts in the field cases . all the guangxi isolates are classified into genotype vii of apmv - 1 , the same genotype dominated in china and other areas in recent years
结果发现，广西分离株之间在信号肚的核旮酸和氨基酸同源性很高，而与其它参考株差异较大；广西分离株在裂解位点的氨基酸组成和排列均符合强毒株的特征，并与毒株在临床上的致病情况相符；根据apmvlf基因第47位第420位核苦酸序列所绘制的系谱树吵ylogenetictree ）来看，厂西鸡和鹅分离株都归属于基因型vll 。 - The n - terminal nucleotides 47 - 420 of the guangxi apmv - 1 isolates of different poultry species origin were amplified and sequenced . the alignment and phylogenetic analysis of the nucleotide sequences and deduced amino acid sequences of f gene of the guangxi isolates and other reference strains obtained from genbank were done
研究对鸡、鹅、鸽三种禽源apmv刁广西分离株f基因n与前段进行了rtpcr扩增及核茸酸序列测定，并用基因分析软件dnastar进行分析并与已发表的其它参考毒株进行比较。 - One pair of specific primers pai1 and pai2 was designed according to the sequence of ha gene of aiv h5 subtype reference strain . the fragment of ha gene was amplified by rt - pcr using its genome rna as template . the obtained target fragment was cloned into the vector pmd18 - t and th en e . coli was transformed
以其基因组为模板，根据禽流感h5亚型参考毒株ha基因序列，设计合成了一对特异性引物pai1和pai2 ，经反转录-聚合酶链式反应( rt - pcr )扩增出本毒株的cdna ，所得片段连接到载体pmd18 - t后，转化大肠杆菌。 - The nucleotide sequences and deduced amino acid sequences of the m gene of 8 ibv isolates in china with the published sequences of reference strains in genbank were analyzed and compared , the results showed that the nucleotide sequences homogeneity between them is 87 . 1 % ~ 100 % , and the deduced amino acid sequences homogeneity is 88 . 1 % ~ 100 %
将m基因核苷酸序列和推导的氨基酸序列进行比较的结果表明ibv分离株彼此间的核苷酸同源性为87 . 1 - 100 ，其相应的氨基酸序列的同源性为88 . 1 - 100 。 - Infectious bursal disease virus has been a great concern for the poultry industry for a long time , particularly for the past decade when its " re - emergence " in variant or highly virulent forms . in this study , two sets of primers ( pta and pts , ibda and ibds ) , flanking the hyper - variable region of vp2 gene , were designed to run a reverse transcription polymerase chain reaction ( primary rt - pcr ) and nested - pcr . both of these assays can amplify all of 12 reference strains which including pathotypes cibdv , vvibdv and vibdv , but not the 5 negative reference pathogens of chicken
结果12个参考毒株均能扩增出约679bp的目的片段，而阴性对照的常见5种鸡病病原：鸡新城疫病毒( ndv ) 、鸡传染性支气管炎病毒( ibv ) 、鸡传染性贫血病毒( caiv ) 、大肠杆菌和多杀性巴氏杆菌均没有扩增到任何片段；应用建立的技术对疑似ibd的34份临床病料进行检测，并同时在基础rt - pcr扩增的片段内设计另一对引物进行nested - pcr 。 - This strain ' s virulence was judged by mean death time of chick embryos ( mdt ) , intracerebral pathogenicity index in day - old chicks ( icpi ) and intravenous pathogenicity index in 6 - week - old chickens ( ivpi ) and it was found to be the virulent strain . at last , it was tested by the recurrent infection and found that it was the newcastle disease virus ( ndv ) , and it was named hbg - 1 strain . in order to find the difference of the cleavage site of this strain with f48e9 and ? 30 strain , a part of the cleavage site of fusion protein gene fragment was amplified by rt - pcr using a primer and sequenced . the sequence analysis showed this strain had low homology with f4ge9 and cso . a phylogenetic tree based on the published sequences of ndv reference strains was constructed and showed the isolated strain hbg - 1 belonged to the genotype w ndv , a novel genotype ndv
为了进一步探寻分离株与标准株的异同，又采用rt - pcr方法，扩增获得分离株f _ o裂解位点附近的基因片段，经测序后与国际上已发表的新城疫病毒的核酸序列进行比较，结果表明其与标准株和疫苗株之间的同源性较低，仅为82 86之间。经系统发育进化树分析后，判定该分离株为新城疫病毒( ndv )基因型。运用计算机软件对其裂解位点处的氨基酸序列进行预测和分析，结果表明该分离株为新城疫病毒强毒株并具有基因型的典型结构特征。
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